Coding

Part:BBa_K1179060:Design

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)


eYFP-4x451ts


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 877
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 877
    Illegal SpeI site found at 853
    Illegal PstI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The miRNA451 doesn't completely repress the eYFP, some background expression is still visible.

Source

This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has been modified to fluoresce yellow and also synthesized with four miRNA451 target sites.

References